explain the terms gene, locus, allele, dominant, recessive, codominant, linkage, test cross, F1, F2, phenotype, genotype, homozygous and heterozygous

Published by Patrick Mutisya · 14 days ago

Cambridge A‑Level Biology 9700 – Roles of Genes in Determining the Phenotype

Objective

Explain the following terms and how they relate to the inheritance of traits:

  • gene
  • locus
  • allele
  • dominant
  • recessive
  • codominant
  • linkage
  • test cross
  • F1 and F2 generations
  • phenotype
  • genotype
  • homozygous
  • heterozygous

Key Definitions

TermDefinition
GeneA segment of DNA that codes for a specific protein or functional RNA.
LocusThe fixed position of a gene on a chromosome.
AlleleOne of two or more alternative forms of a gene that arise by mutation and are found at the same locus.
DominantAn allele whose phenotype is expressed in both homozygous and heterozygous individuals.
RecessiveAn allele whose phenotype is expressed only when an individual is homozygous for that allele.
CodominantTwo alleles at a locus are both fully expressed in the heterozygote, giving a phenotype that shows both traits.
LinkageThe tendency of genes that are close together on the same chromosome to be inherited together.
Test crossA cross between an individual of unknown genotype and a homozygous recessive individual to determine the unknown genotype.
F1 generationThe first filial generation produced by crossing two parental (P) individuals.
F2 generationThe second filial generation obtained by inter‑crossing individuals from the F1 generation.
PhenotypeThe observable characteristics of an organism resulting from the interaction of its genotype with the environment.
GenotypeThe genetic constitution of an organism at a particular locus (e.g., AA, Aa, aa).
HomozygousHaving two identical alleles at a locus (AA or aa).
HeterozygousHaving two different alleles at a locus (Aa).

Monohybrid Cross Example

Consider a single gene with two alleles: A (dominant) and a (recessive). The parental cross is:

\$\$

\begin{array}{c|c}

\text{Parent 1 (AA)} & \text{Parent 2 (aa)} \\

\hline

\text{Gametes: A} & \text{Gametes: a}

\end{array}

\$\$

The F1 offspring are all heterozygous (Aa) and display the dominant phenotype.

F2 Generation

Inter‑crossing two F1 individuals (Aa × Aa) gives the classic 3:1 phenotypic ratio.

\$\$

\begin{array}{c|cc}

& A & a \\ \hline

A & AA & Aa \\

a & Aa & aa

\end{array}

\$\$

Genotypic ratio: 1 AA : 2 Aa : 1 aa

Phenotypic ratio (dominant : recessive): 3 : 1

Test Cross

To determine whether an individual with a dominant phenotype is homozygous (AA) or heterozygous (Aa), cross it with a homozygous recessive (aa) individual.

  1. If the unknown parent is AA, all offspring will be Aa (dominant phenotype).
  2. If the unknown parent is Aa, the offspring will be ½ Aa (dominant) and ½ aa (recessive), giving a 1:1 phenotypic ratio.

Linkage and Recombination

When two genes are on the same chromosome, they may be inherited together. The degree of linkage is expressed by the recombination frequency (θ), calculated as:

\$\$

\theta = \frac{\text{Number of recombinant offspring}}{\text{Total number of offspring}} \times 100\%

\$\$

A recombination frequency of 0 % indicates complete linkage; 50 % indicates independent assortment.

Codominance Example

In the ABO blood‑group system, alleles IA and IB are codominant. An individual with genotype IAIB expresses both A and B antigens on the surface of red blood cells.

Suggested diagram: Punnett square for a monohybrid cross (AA × aa) and a test cross (Aa × aa).

Summary Checklist

  • Identify the locus and alleles involved in a trait.
  • Determine whether alleles are dominant, recessive, or codominant.
  • Predict F1 and F2 phenotypic ratios using Punnett squares.
  • Use a test cross to reveal hidden heterozygosity.
  • Consider linkage when genes are on the same chromosome; calculate recombination frequency.