Lesson Plan

• Describe how temperature affects kinetic energy and collision frequency of enzyme‑substrate molecules.
• Explain the relationship between temperature, enzyme‑substrate fit, and the proportion of effective collisions.
• Analyse why enzymes denature at high temperatures and predict the impact on reaction rates.
• Apply the concepts to interpret a temperature‑activity graph for catalase.

• Projector and screen
• Whiteboard and coloured markers
• Printed worksheet with temperature‑activity table
• Catalase‑hydrogen peroxide demonstration kit (test tubes, water bath, thermometer)
• Exit‑ticket cards

Begin with a quick video of a chef heating a sauce to illustrate how temperature can speed up or ruin a process. Ask students what they already know about enzymes and temperature. State that by the end of the lesson they will be able to explain the molecular reasons behind the optimum temperature and predict what happens when it is exceeded.

1. Do‑now (5') – Label a diagram of an enzyme‑substrate complex and note what might change with temperature.
2. Mini‑lecture (10') – Explain kinetic energy, the Arrhenius equation, and how temperature raises collision frequency.
3. Interactive simulation (8') – Students adjust a temperature slider and observe changes in reaction rate on a graph.
4. Practical demonstration (12') – Conduct the catalase‑hydrogen peroxide experiment at three temperatures; record volume of O₂ produced.
5. Data analysis (10') – Groups plot their results, identify the optimum temperature, and discuss the shape of the curve.
6. Concept check (5') – Quick clicker quiz on effective collisions and denaturation.
7. Summary & exit ticket (5') – Students write one key point they learned and hand in an exit ticket.

Recap the four ways temperature influences enzyme activity and highlight the characteristic peak in the activity‑temperature graph. Students complete an exit ticket answering: “What would happen to enzyme activity if the temperature rises 10 °C above the optimum?” Finally, assign a short homework: sketch a temperature‑activity curve for a different enzyme and label the optimum temperature.