Lesson Plan

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Lesson Plan
Grade: Date: 25/02/2026
Subject: Biology
Lesson Topic: outline the Krebs cycle, explaining that oxaloacetate (4C) acts as an acceptor of the 2C fragment from acetyl coenzyme A to form citrate (6C), which is converted back to oxaloacetate in a series of small steps
Learning Objective/s:
  • Describe the overall reaction and purpose of the Krebs cycle.
  • Explain how oxaloacetate acts as an acceptor for acetyl‑CoA to form citrate.
  • Identify each enzymatic step and the associated cofactors produced.
  • Interpret the net yield of NADH, FADH₂ and GTP per turn of the cycle.
  • Relate the location of the cycle to cellular respiration.
Materials Needed:
  • Projector or interactive whiteboard
  • Slide deck with a detailed Krebs cycle diagram
  • Handout of the step‑by‑step table
  • Molecular model kits (optional) for visualising intermediates
  • Worksheets for labeling the cycle
  • Clickers or online quiz tool for formative checks
 Introduction:
Begin with a quick think‑pair‑share: where does the energy from glucose finally end up? Review that glycolysis produces pyruvate, which enters the mitochondrion as acetyl‑CoA. State that by the end of the lesson students will be able to trace each step of the Krebs cycle and explain the role of oxaloacetate.
Lesson Structure:
  1. Do‑now (5’) – Students label a blank Krebs cycle diagram on the worksheet.
  2. Mini‑lecture (10’) – Present the overall reaction and the role of oxaloacetate using slides.
  3. Guided walkthrough (15’) – Step‑by‑step explanation of each enzyme; students fill a table in their handout.
  4. Interactive modelling (10’) – Use molecular kits or a digital simulation to visualize substrate‑product changes.
  5. Formative check (5’) – Clicker quiz on cofactors and energy yield.
  6. Summary discussion (5’) – Students summarise the cycle in their own words.
Conclusion:
Recap the sequence from oxaloacetate to citrate back to oxaloacetate and the net production of NADH, FADH₂ and GTP. Have students write one key fact on an exit ticket. Assign homework to create a concise flow‑chart of the cycle for revision.