Lesson Plan

• Describe the three main ways genes can be obtained for recombinant work: DNA extraction, cDNA synthesis, and chemical synthesis.
• Explain the key steps and advantages/limitations of each gene‑acquisition method.
• Compare the methods to select the most appropriate one for a given experimental goal.
• Apply verification techniques (restriction analysis, PCR, sequencing) to confirm successful gene acquisition.

• Projector and screen
• PowerPoint/Google Slides presentation
• Handouts with flow‑chart of the three pathways
• Worksheet for step‑labeling activity
• Access to an online gene‑design tool (e.g., Benchling)
• Short video clip showing reverse transcription
• Exit‑ticket slips or digital form

Begin with a quick “What is a gene?” poll to activate prior knowledge. Show a striking image of a genetically modified plant and ask students to guess how the new gene was obtained. State today’s success criteria: students will be able to list and compare the three gene‑acquisition methods and justify their choice for a given scenario.

1. Do‑now (5'): 3‑question quiz on basic gene concepts; collect responses.
2. Mini‑lecture (10'): Overview of DNA extraction method with annotated diagram.
3. Guided practice (10'): Students label the four steps of DNA extraction on the handout; teacher checks understanding.
4. Video demonstration (5'): Short clip of reverse transcription to illustrate cDNA synthesis.
5. Group activity (10'): Using an online tool, groups design a short synthetic gene and discuss codon optimisation.
6. Comparison chart (5'): Whole‑class fill‑in of advantages/limitations table for the three methods.
7. Exit ticket (5'): Students write which method they would choose for a plant‑resistance gene and why.

Recap the three pathways and emphasise that verification is essential regardless of the source. Collect exit tickets as a formative check and assign a short homework: research a real‑world GMO and identify which gene‑acquisition method was used.